Proteomic analysis of low-molecular-weight glutenin subunits and relationship with their genes in a common wheat variety
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چکیده
منابع مشابه
Allelic analysis of low molecular weight glutenin subunits using 2-DGE in Korean wheat cultivars
Two-dimensional gel electrophoresis (2-DGE) was used as a complement to SDS-PAGE to determine the allelic compositions of LMW-GS in 32 Korean wheat cultivars. Protein patterns generated by 2-DGE from each cultivar were compared to patterns from standard wheat cultivars for each allele. At the Glu-A3 locus, thirteen c, twelve d, three e (null), two g and two new alleles were identified. At the G...
متن کامل[Cloning and phylogenetic analysis of low-molecular-weight glutenin subunit genes at Glu-B3 locus in common wheat relative species].
The common wheat relative species are important germplasm for wheat breeding. In the present study, novel allelic variants at Glu-B3 locus were cloned to provide gene resources for wheat quality improvement. Four Glu-B3-locus specific primer sets LB1F/LB1R, LB2F/LB2R, LB3F/LB3R, and LB4F/LB4R were employed to isolate novel allelic variants of GluB3-1, GluB3-2, GluB3-3, and GluB3-4 from seven co...
متن کاملConserved cis-regulatory modules in promoters of genes encoding wheat high-molecular-weight glutenin subunits
The concentration and composition of the gliadin and glutenin seed storage proteins (SSPs) in wheat flour are the most important determinants of its end-use value. In cereals, the synthesis of SSPs is predominantly regulated at the transcriptional level by a complex network involving at least five cis-elements in gene promoters. The high-molecular-weight glutenin subunits (HMW-GS) are encoded b...
متن کاملidentification of low molecular weight glutenin subunits in triticum aestivum and the d genome of wild related wheat
in this study, 110 samples of bread wheat, aegilops crassa, ae. cylindrica and ae. tauschii were investigated to identify low-molecular-weight glutenin subunits. overall, the four alleles in term of the analysis of sds-page gels were detected. the ʻaʼ allele with 45% has the greatest frequency, and after that, ʻbʼ, ʻcʼ and ʻdʼ alleles were with 38.1%, 11.8% and 9.1% frequencies, respectively; h...
متن کاملCellular Localization of Wheat High Molecular Weight Glutenin Subunits in Transgenic Rice Grain
Rice (Oryza sativa L.) is a primary global food cereal. However, when compared to wheat, rice has poor food processing qualities. Dough that is made from rice flour has low viscoelasticity because rice seed lacks storage proteins that are comparable to gluten protein from wheat. Thus, current research efforts aim to improve rice flour processing qualities through the transgenic expression of vi...
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ژورنال
عنوان ژورنال: 3 Biotech
سال: 2018
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-017-1081-2